PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

The P38 mitogen-activated protein kinase (MAPK) signal transduction pathway is widespread in organisms and plays important roles immune activities. infection mechanism of environmental gram-negative bacteria on crustaceans an scientific problem. In this study, the cDNA full-length sequence Portunus trituberculatus (PtP38) was cloned its structure analyzed by bioinformatics methods. To study function PtP38 gene after a Gram-negative bacterial infection, we injected P. with LPS to activate response instead directly infecting bacteria. With stimulation, expression different tissues increased significantly. At same time, immune-related genes (ALF crustin) hepatopancreas, activities antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), inducible nitric oxide synthase (iNOS) enzymes], apoptosis-related (caspase2 caspase3) were further conform response, inhibitor subsequently LPS. results showed that inhibited, activity decreased, inhibited. Thus, speculated may increase ability improving antimicrobial peptides, increasing oxidative stress-related enzymes, promoting cell apoptosis infected . This also laid foundation for MAPK signaling